Journal: Materials today. Bio

Article Title: Precise delivery of doxorubicin and imiquimod through pH-responsive tumor microenvironment-active targeting micelles for chemo- and immunotherapy.

doi: 10.1016/j.mtbio.2022.100482

Figure Lengend Snippet: Fig. 10. Immunostaining of tumor tissues in tumor-bearing mice after treatments. (A) Immunohistochemistry images of tumor sections stained with CD3, CD8, and TNF-α antibodies. The scale bar is 100 μm. (B) Immunofluorescence images of tumor tissues after treatments for 12 days. The scale bar is 50 μm. Blue fluorescence represents the cell nucleus stained with DAPI. Green fluorescence represents the iNOS stained with the iNOS antibody conjugated with FITC.

Article Snippet: After 30 min, the tissue slice was stained with diluted iNOS antibody (Miltenyi Biotec, catalog: 130-116-357) at 4 C overnight.

Techniques: Immunostaining, Immunohistochemistry, Staining

Journal: Neurotherapeutics

Article Title: Down-regulation of lipocalin-2 alleviates depressive-like behaviors in mice through modulation of microglial activation

doi: 10.1016/j.neurot.2026.e00862

Figure Lengend Snippet: LCN2 neutralizing antibodies alleviated the depressive-like behaviors in CUS mice. ( A ) Schematic of the experimental procedure and behavioral studies. ( B ) Levels of LCN2 measured by ELISA in the mouse hippocampus treated with IgG or anti-LCN2 (n = 6/group). ∗∗∗ P < 0.001 versus the IgG group using Student's t -test. ( C – H ) LCN2 neutralizing antibodies relieved depressive-like behaviors in CUS mice (n = 12/group) as measured by the SPT ( C ), FST ( D) , TST ( E ) and OFT ( F – H ). ∗∗∗ P < 0.001 versus the Control + IgG group; ### P < 0.001 versus the CUS + anti-IgG group using two-way ANOVA followed by the Holm-Sidak post hoc multiple comparison test.

Article Snippet: After incubation in blocking buffer, membranes were incubated overnight at 4 °C with the following primary antibodies: anti-LCN2 (1:1000, abs134038, Absin), anti-iNOS (1:1000, 18985-1-AP, Proteintech Group), anti-p(Ser616)DRP1 (1:1000, ab314755, Abcam), anti-DRP1 (1:1000, 12957-1-AP, Proteintech Group), anti-MFN2(1:1000, 12186-1-AP, Proteintech Group), and anti-GAPDH (1:3000, 60004-1-AP, Proteintech Group).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison

Journal: Neuromolecular medicine

Article Title: Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway.

doi: 10.1007/s12017-018-8495-9

Figure Lengend Snippet: Fig. 6 Inhibitory effect of PL on LPS-induced amyloidogen- esis and neuroinflammation in cultured brain cells. The expres- sions of amyloidogenesis pro- teins, such as APP and BACE1, were detected by Western blot- ting using specific antibodies in the astrocyte primary (a) and microglial BV-2 cells (b). The levels of Aβ1−42 in the astrocyte primary (c) and microglial BV-2 cells (d) were measured by ELISA. Data are expressed as the mean ± S.E.M. (t test, *p < .05 vs. control, #p < .05 vs. LPS). The expressions of neuro- inflammation proteins, such as iNOS, COX-2 and GFAP, were detected by Western blotting using specific antibodies in the astrocyte primary (e) in the microglial BV-2 cells (f). Each blot is representative of three experiments

Article Snippet: The sections were reacted with specific primary antibodies for Aβ (1:300; Abcam, Inc., Cambridge, MA, USA), glial fibrillary acidic protein (GFAP) (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), ionize calcium-binding adapter molecule 1 (IBA-1) (1:300; Abcam, Inc., Cambridge, MA, USA), inducible nitric oxide synthase (iNOS) (1:300; Novus Biologicals, Inc., Littleton) and cyclooxygenase-2 (COX-2) (1:300; Cell Signaling Technology, Inc., Beverly, MA, USA).

Techniques: Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Italian Journal of Animal Science

Article Title: Protective effect of chitosan oligosaccharide against oxidative damage of peripheral blood mononuclear cells in dairy cows induced by diethylenetriamine/nitric oxide via NF-κB signalling pathway

doi: 10.1080/1828051x.2020.1772131

Figure Lengend Snippet: Figure 2. Effects of chitosan oligosaccharideon on the phosphorylation level of NF-kB pathway, the protein expression level of nitric oxide synthase (iNOS) and interleukin-1b (IL-1b). Expressions of IL-1b(A); phosphorylated IjB kinase b (P-IKKb) (B); phosphorylated inhibitor of nuclear factor kappa-Ba (P-IjBa) (C); phosphorylated nuclear factor kappa-Bp65(P-NF-jBp65) (D); iNOS (E), protein levels were detected by western blotting and normalised to beta-actin (b-Actin) levels. CTR¼ control treatment, without chitosan oligosacchar- ides addition; COS40, COS80, COS160, and COS320¼ treated with 40, 80, 160, and 320lg/mL chitosan oligosaccharides respectively.

Article Snippet: The membranes were incubated in the following primary antibodies: primary polyclonal rabbit anti-iNOS antibody (NBP1-97471, Novus Biological, USA), rabbit anti-IL-1b (Cell Signalling Technology, Boston, MA), anti-IKKb, monoclonal rat anti-IjBa, monoclonal rabbit anti-NF-jBp65 antibody(NF-jB pathway Sampler Kit 9963,CST, MA), anti-phospho-IKKb, anti-phospho-IjBa, and anti-phospho-NF-jBp65 (NF-jB pathway Sampler Kit 9963, CST, MA).

Techniques: Phospho-proteomics, Expressing, Western Blot, Control